Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Fgf18 and the recovery rates were calculated by comparing the measured value to the expected amount of Fgf18 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
92
EDTA plasma(n=5)
85-103
96
heparin plasma(n=5)
85-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fgf18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-104%
87-100%
85-101%
EDTA plasma(n=5)
85-101%
85-100%
82-99%
heparin plasma(n=5)
88-100%
83-99%
81-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
O88182
Applications
ELISA
Applications Notes
Fgf18ELISA Kit allows for the in vitro quantitative determination of Fgf18 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Fgf18 (Fibroblast growth factor 18) ELISA Kit