Rat FGF1(Fibroblast Growth Factor 1, Acidic) ELISA Kit

Mfr.No.

ELK2485

Sensitivity

6.1 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Rat

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta

Recovery

Matrices listed below were spiked with certain level of recombinant FGF1 and the recovery rates were calculated by comparing the measured value to the expected amount of FGF1 in samples.

Matrix	Recovery range	Average
serum(n=5)	89-103%	96%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	80-95%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	89-98%	83-103%	98-105%	92-101%
EDTA plasma(n=5)	92-101%	87-98%	85-92%	79-96%
Heparin plasma(n=5)	86-95%	85-96%	89-100%	85-96%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.121	2.033
500.00	1.643	1.555
250.00	1.135	1.047
125.00	0.782	0.694
62.50	0.548	0.460
31.25	0.315	0.227
15.63	0.202	0.114
0.00	0.088	0.000

Limit Of Detection

1000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FGF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P61149

Applications	ELISA
Research Area	Cytokine;Tumor immunity;Infection immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15205465	ELK2485	48T	$320		Add to Cart
BHE15205465	ELK2485	96T	$458		Add to Cart

Rat FGF1(Fibroblast Growth Factor 1, Acidic) ELISA Kit

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