Rat FABP3 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ1623

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.075	0.166	0.229	0.356	0.588	1.016	1.722	2.411

Description

Rat FABP3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat Fabp3 in cell culture supeatants, serum and plasma (heparin, EDTA).

Alternative Names

Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Fabp3

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-A133

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat FABP3

Subcellular Localization

Cytoplasm.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	244	986	4588	267	1042	4602
Standard deviation	9.52	48.31	261.52	11.21	53.14	289.93
CV (%)	3.9%	4.9%	5.7%	4.2%	5.1%	6.3%

Protein Function

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

Background

Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	79131
Gene Name	Fabp3
UniProt ID	P07483
Tissue Specificity	Heart, but also skeletal muscle, kidney, brain and mammary gland.

Applications	ELISA
Research Area	Cardiovascular,Fatty Acid Oxidation,Fatty Acids,Lipid and Lipoprotein Metabolism,Lipids/Lipoproteins,Mesenchymal Stem Cells,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Redox Metabolism,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000054	EZ1623	5 plates/kit	$500		Add to Cart

Rat FABP3 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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