Rat F12 (Coagulation Factor XII) CLIA Kit

Mfr.No.

E-CL-R0155

Sensitivity

75 pg/mL

Detection Range

125-8000 pg/mL

Reactivity

Rat

Assay Duration

3.5h

Assay Type

Sandwich

Description

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat F12. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F12, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat F12. You can calculate the concentration of Rat F12 in the samples by comparing the RLU value of the samples to the standard curve.

Detection Method

Chemiluminescence

Recovery

80%-120%

Linearity

Samples were spiked with high concentrations of Rat FⅫ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	96-107	93-108	86-96
1:2	Average (%)	101	100	91
1:4	Range (%)	99-116	94-109	98-112
1:4	Average (%)	106	102	106
1:8	Range (%)	98-109	94-106	95-109
1:8	Average (%)	104	100	102
1:16	Range (%)	96-108	104-118	88-100
1:16	Average (%)	102	110	93

Specificity

This kit recognizes Rat F12 in samples.No significant cross-reactivity or interference between Rat F12 and analogues was observed

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Precision

Both intra-CV and inter-CV are < 10%.

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Usage Notes

This ELISA kit applies to the in vitro quantitative determination of Rat F12 concentrations in Serum, plasma and other biological fluids.

UniProt ID	D3ZTE0

Applications	CLIA
Research Area	Cancer, Cardiovascular

Storage	2-8°C/-20°C,6 months
Shipping	Ice bag

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SKU	Supplier No.	Size	Your price	Quantity
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Rat F12 (Coagulation Factor XII) CLIA Kit

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