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Home / ELISA Kit

Rat extracellular superoxide dismutase [Cu-Zn](Cu/Zn-SOD/SOD3)ELISA kit

SKU : BHE10507946

This Rat SLC2A4 ELISA Kit was designed for the quantitative measurement of Rat SLC2A4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E14981r

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

superoxide dismutase 3, extracellular, EC-SOD, MGC20077

Recovery

The recovery of rat GLUT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GLUT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		87
		Range %		80-92
1:2		Average %		98
		Range %		91-105
1:4		Average %		101
		Range %		92-110
1:8		Average %		93
		Range %		86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.958	3.000	2.979	2.850
5	2.461	2.460	2.461	2.332
2.5	1.852	1.876	1.864	1.735
1.25	1.234	1.261	1.248	1.119
0.625	0.753	0.798	0.776	0.647
0.312	0.531	0.578	0.555	0.426
0.156	0.396	0.412	0.404	0.275
0	0.132	0.126	0.129	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

UniProt ID	Q08420

Applications	ELISA
Research Area	SOD3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10507946	CSB-E14981r	96T	$595		Add to Cart
BHE10507946	CSB-E14981r	5x96T	$2856		Add to Cart
BHE10507946	CSB-E14981r	10x96T	$5652.50		Add to Cart

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