rat Estradiol receptor,ER ELISA Kit

rat Estradiol receptor,ER ELISA Kit

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Mfr.No.

CSB-E06848r

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

estrogen receptor 1, RP1-130E4.1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1, estrogen receptor alpha, estrogen receptor alpha delta 3*,4,5,6,7*/819-2 isoform, estrogen receptor alpha delta 4 +49 isoform, estrogen receptor

Recovery

The recovery of human AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-90
1:2	Average %	94
	Range %	90-98
1:4	Average %	87
	Range %	83-90
1:8	Average %	100
	Range %	95-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.392	2.375	2.384	2.222
10	1.859	1.804	1.832	1.670
5	1.411	1.435	1.423	1.261
2.5	0.966	0.968	0.967	0.805
1.25	0.540	0.568	0.554	0.392
0.625	0.363	0.368	0.366	0.204
0.312	0.235	0.241	0.238	0.076
0	0.164	0.160	0.162

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P06211

Applications	ELISA
Research Area	ESR1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10507941	CSB-E06848r	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.