Matrices listed below were spiked with certain level of ERK2 and the recovery rates were calculated by comparing the measured value to the expected amount of ERK2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-99
94
EDTA plasma(n=5)
86-100
90
heparin plasma(n=5)
85-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERK2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ERK2 ELISA Kit allows for the in vitro quantitative determination of ERK2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat ERK2(Extracellular Signal Regulated Kinase 2) ELISA Kit