Matrices listed below were spiked with certain level of ERK1/2 and the recovery rates were calculated by comparing the measured value to the expected amount of ERK1/2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
98
EDTA plasma(n=5)
86-100
94
heparin plasma(n=5)
91-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERK1/2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ERK1/2 ELISA Kit allows for the in vitro quantitative determination of ERK1/2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit