Rat Epidermal growth factor,EGF ELISA Kit

Mfr.No.

CSB-E08029r

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Epidermal growth factor,EGF

Recovery

The recovery of mouse TNFsR-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	98	95-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFsR-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	91
	Range %	86-95
1:40	Average %	97
	Range %	92-102
1:80	Average %	90
	Range %	87-98
1:160	Average %	92
	Range %	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P07522

Applications	ELISA
Research Area	EGF

Storage	Store at 2-8°C. Please refer to protocol.

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