Rat eosinophil cationic protein,ECP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E11238r

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ribonuclease, RNase A family, 3 (eosinophil cationic protein), ECP, RNS3, cytotoxic ribonuclease, eosinophil cationic protein

Recovery

The recovery of rat CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	96-104
EDTA plasma (n=4)	93	89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	100
	Range %	98-103
1:2	Average %	96
	Range %	92-100
1:4	Average %	92
	Range %	87-98
1:8	Average %	89
	Range %	84-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P70709

Applications	ELISA
Research Area	RNASE3

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. Seiffert J et al,PLoS One,2015 Mar 6;10(3):e0119726.

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