Rat Endothelin 1 - ET-1 ELISA Kit

Mfr.No.

E0462Ra

Alternative Names

Big endothelin-1;EDN 1;EDN1;Endothelin-1;ET-1;PPET1;Preproendothelin-1

Reactivity

Rat

Detection Range

2-600ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

23135

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.02ng/L

Background

Endothelins are endothelium-derived vasoconstrictor Peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (By similarity).

Gene ID	24323
UniProt ID	P22388
Antigen	EDN1

Research Area

Cancer

Protocol

These standard curves of E0462Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	2.223	2.246	2.209
	2.269
160ng/L	1.553	1.484	1.447
	1.415
80ng/L	0.678	0.64	0.603
	0.602
40ng/L	0.361	0.346	0.309
	0.331
20ng/L	0.167	0.173	0.136
	0.18
0ng/L	0.044	0.037	0
	0.031

Precision

We measured random samples of E0462Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	147.6	4.89	3.3
Intra-Assay	2	18	51.5	2.02	3.9
Intra-Assay	3	18	85.5	3.6	4.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111750	E0462Ra	48T	$320		Add to Cart
BHE12111750	E0462Ra	96T	$458		Add to Cart

Rat Endothelin 1 - ET-1 ELISA Kit

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