Rat Endostatin,ES ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07975r

Sensitivity

1.56 ng/mL

Detection Range

6.25 ng/mL-400 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Endostatin,ES, FLJ27325, FLJ34914, KNO, KNO1, MGC74745, alpha 1 type XVIII collagen, antiangiogenic agent, endostatin, multi-functional protein MFP

Recovery

The recovery of pig SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-99
EDTA plasma (n=4)	103	98-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	92-103
1:2000	Average %	95
	Range %	91-99
1:4000	Average %	101
	Range %	98-105
1:8000	Average %	95
	Range %	86-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	ES

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of 8 weeks endurance training on serum vascular endothelial growth factor and endostatin in Wistar rats Maryam Nourshahi et al,Koomesh ,2012 13(4): 474-479 Effect of Niacin on Inflammation and Angiogenesis in a Murine Model of Ulcerative Colitis Salem HA.et al,Sci Rep,Aug 2

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