Matrices listed below were spiked with certain level of ELAV1 and the recovery rates were calculated by comparing the measured value to the expected amount of ELAV1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
98
EDTA plasma(n=5)
90-103
96
heparin plasma(n=5)
87-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ELAV1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-103%
87-97%
88-96%
EDTA plasma(n=5)
85-96%
86-97%
87-98%
heparin plasma(n=5)
84-100%
81-91%
85-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
B5DF91
Antigen
ELAV1
Applications
ELISA
Applications Notes
ELAV1 ELISA Kit allows for the in vitro quantitative determination of ELAV1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat ELAV1(ELAV-like protein 1) ELISA Kit