Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Eif2ak3 and the recovery rates were calculated by comparing the measured value to the expected amount of Eif2ak3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
94
EDTA plasma(n=5)
87-105
97
heparin plasma(n=5)
88-104
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Eif2ak3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-98%
94-103%
85-104%
EDTA plasma(n=5)
82-98%
86-97%
85-96%
heparin plasma(n=5)
86-100%
82-98%
84-100%
Sensitivity
< 28.125pg/ml
Background
PERK,PRKR-like endoplasmic reticulum kinase,
Notes
For Research Use Only
UniProt ID
Q9Z1Z1
Antigen
EIF2AK3
Applications
ELISA
Applications Notes
Eif2ak3 ELISA Kit allows for the in vitro quantitative determination of Eif2ak3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Eif2ak3 (Eukaryotic translation initiation factor 2-alpha kinase 3) ELISA Kit