Rat Dihydrotestosterone,DHT ELISA Kit

Rat Dihydrotestosterone,DHT ELISA Kit

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Mfr.No.

CSB-E07879r

Alternative Names

Dihydrotestosterone,DHT

Reactivity

Rat

Detection Range

10 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-106
EDTA plasma (n=4)	98	91-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	95
	Range %	90-100
1:2000	Average %	94
	Range %	91-99
1:4000	Average %	92
	Range %	88-98
1:8000	Average %	93
	Range %	84-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.754	2.797	2.776	2.564
500	2.312	2.354	2.333	2.121
250	1.563	1.554	1.559	1.347
125	0.735	0.753	0.744	0.532
62.5	0.538	0.526	0.532	0.320
31.2	0.322	0.335	0.329	0.117
15.6	0.268	0.284	0.276	0.064
0	0.211	0.213	0.212	?

Species

Rattus norvegicus

Sensitivity

5 pg/mL

Limit Of Detection

450 nm

Antigen	Dihydrotestosterone,DHT

Applications

ELISA

Research Area

DHT

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Cynanchum wilfordii Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia by Regulating 5a-Reductase and Androgen Receptor Activities in a Rat Model Lee G.et al,Nutrients,Sep 27 Additive effect of oral LDD175 to tamsulosin and finasteride in a benign prostate hyperplasia rat model Bo Ram Choi.et al,Drug Des Devel Ther,June 22 Seasonal expression of 5α-reductases and androgen receptor in the prostate gland of the wild ground squirrel (Spermophilus dauricus) YanZhang.et al,COMP BIOCHEM PHYS A,July 2 Prenatal exposure to testosterone induces cardiac hypertrophy in adult female rats through enhanced Pkcδ expression in cardiac myocytes Hou M, et al,Journal of Molecular and Cellular Cardiology,January 11

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SKU	Supplier No.	Size	Your price
BHE10507890	CSB-E07879r	96T	$856	Add to Cart
BHE10507890	CSB-E07879r	5x96T	$4108.80	Add to Cart
BHE10507890	CSB-E07879r	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.