Rat Dihydrotestosterone,DHT ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07879r

Sensitivity

5 pg/mL

Detection Range

10 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Dihydrotestosterone,DHT

Recovery

The recovery of human fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-106
EDTA plasma (n=4)	98	91-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	95
	Range %	90-100
1:2000	Average %	94
	Range %	91-99
1:4000	Average %	92
	Range %	88-98
1:8000	Average %	93
	Range %	84-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	DHT

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Cynanchum wilfordii Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia by Regulating 5a-Reductase and Androgen Receptor Activities in a Rat Model Lee G.et al,Nutrients,Sep 27 Additive effect of oral LDD175 to tamsulosin and finasteride in a benign prostate hyperplasia rat model Bo Ram Choi.et al,Drug Des Devel Ther,June 22 Seasonal expression of 5α-reductases and androgen receptor in the prostate gland of the wild ground squirrel (Spermophilus dauricus) YanZhang.et al,COMP BIOCHEM PHYS A,July 2 Prenatal exposure to testosterone induces cardiac hypertrophy in adult female rats through enhanced Pkcδ expression in cardiac myocytes Hou M, et al,Journal of Molecular and Cellular Cardiology,January 11

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