Rat diamine oxidase(DAO) ELISA Kit

Mfr.No.

CSB-E12634r

Alternative Names

D-amino-acid oxidase, DAAO, DAMOX, MGC35381, OXDA

Reactivity

Rat

Detection Range

0.78 mIU/mL-50 mIU/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat ARRB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	92-106
EDTA plasma (n=4)	100	94-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ARRB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	104
	Range %	90-109
1:2	Average %	101
	Range %	91-105
1:4	Average %	97
	Range %	88-100
1:8	Average %	104
	Range %	94-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

0.195 mIU/mL

Limit Of Detection

450 nm

UniProt ID	O35078
Antigen	D-amino-acid oxidase

Applications

ELISA

Research Area

DAO

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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