Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of DBP and the recovery rates were calculated by comparing the measured value to the expected amount of DBP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
98
EDTA plasma(n=5)
88-104
94
heparin plasma(n=5)
89-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DBP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
DBP ELISA Kit allows for the in vitro quantitative determination of DBP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat DBP (Vitamin D Binding Protein) ELISA Kit