Rat cytochrome P450 2E1,CYP2E1 ELISA Kit

Rat cytochrome P450 2E1,CYP2E1 ELISA Kit

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Mfr.No.

CSB-E09782r

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

cytochrome P450, family 2, subfamily E, polypeptide 1, CPE1, CYP2E, P450-J, P450C2E, cytochrome P450 2E1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, flavoprotein-linked monooxygenase, microsomal monooxygenase, xenobiotic monooxygenas

Recovery

The recovery of mouse AGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	92	85-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-101
1:2	Average %	95
	Range %	89-100
1:4	Average %	93
	Range %	87-99
1:8	Average %	94
	Range %	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.594	2.568	2.581	2.404
500	1.925	1.899	1.912	1.735
250	1.213	1.178	1.196	1.019
125	0.807	0.789	0.798	0.621
62.5	0.454	0.424	0.439	0.262
31.2	0.317	0.302	0.310	0.133
15.6	0.264	0.254	0.259	0.082
0	0.178	0.176	0.177	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P05182

Applications	ELISA
Research Area	CYP2E1

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10507873	CSB-E09782r	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.