Rat Cytochrome P450 19A1(CYP19A1) ELISA kit

Rat Cytochrome P450 19A1(CYP19A1) ELISA kit

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Mfr.No.

CSB-EL006394RA

Alternative Names

cytochrome P450, family 19, subfamily A, polypeptide 1, ARO, ARO1, CPV1, CYAR, CYP19, MGC104309, P-450AROM, OTTHUMP00000162543, aromatase, cytochrome P450, subfamily XIX (aromatization of androgens), estrogen synthetase, flavoprotein-linked monooxygenase, mic

Reactivity

Rat

Detection Range

18.75 pg/mL-1200 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat Nephrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	97-105
EDTA plasma (n=4)	98	95-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Nephrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	95
	Range %	91-98
1:4	Average %	95
	Range %	81-89
1:8	Average %	100
	Range %	97-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.215	2.194	2.205	2.049
40	1.846	1.804	1.825	1.669
20	1.444	1.468	1.456	1.300
10	1.033	1.068	1.051	0.895
5	0.634	0.671	0.653	0.497
2.5	0.373	0.398	0.386	0.230
1.25	0.297	0.298	0.298	0.142
0	0.154	0.157	0.156

Species

Rattus norvegicus

Sensitivity

4.68 pg/mL

Limit Of Detection

450 nm

UniProt ID	P22443
Antigen	cytochrome P450, family 19, subfamily A, polypeptide 1

Applications

ELISA

Research Area

CYP19A1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507870	CSB-EL006394RA	96T	$695	Add to Cart
BHE10507870	CSB-EL006394RA	5x96T	$3336	Add to Cart
BHE10507870	CSB-EL006394RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.