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Home / ELISA Kit

Rat Cytochrome B5 Type B - CYB5B ELISA Kit

SKU : BHE12112940

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.

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Bioassay Technology Laboratory

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Mfr.No.

E1734Ra

Sensitivity

5.86ng/L

Detection Range

10-3000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CYB 5B;CYB5B;Cytochrome b5 outer mitochondrial membrane isoform;Cytochrome b5 type B

Molecular Weight

16265

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1734Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1356.1	51.44	3.8
Intra-Assay	2	18	856.5	46.52	5.4
Intra-Assay	3	18	555.4	17.37	3.1

Background

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.

Protocol

These standard curves of E1734Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.668	1.821	1.778
	1.974
800ng/L	1.138	1.09	1.048
	1.043
400ng/L	0.454	0.433	0.39
	0.411
200ng/L	0.251	0.261	0.218
	0.271
100ng/L	0.134	0.129	0.087
	0.125
0ng/L	0.027	0.043	0
	0.058

Gene ID	80773
UniProt ID	P04166

Research Area	Tags & Cell Markers

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112940	E1734Ra	48T	$320		Add to Cart
BHE12112940	E1734Ra	96T	$458		Add to Cart

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