Rat Cystatin-S - CST4 ELISA Kit

Mfr.No.

E2454Ra

Alternative Names

Cst4;Cystatin-1;Cystatin-S;Protein LM

Reactivity

Rat

Detection Range

0.2-60ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

15949

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.12ng/ml

Background

This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.

Gene ID	296234
UniProt ID	P19313
Antigen	CST4

Research Area

Others

Protocol

These standard curves of E2454Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.008	1.988	1.943
	1.967
16ng/ml	1.124	1.056	1.011
	0.989
8ng/ml	0.489	0.462	0.417
	0.435
4ng/ml	0.298	0.243	0.198
	0.188
2ng/ml	0.083	0.075	0.03
	0.067
0ng/ml	0.03	0.045	0
	0.06

Precision

We measured random samples of E2454Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	20.49	0.55	2.67
Intra-Assay	2	18	8.25	0.36	4.4
Intra-Assay	3	18	18.46	0.77	4.16

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115439	E2454Ra	48T	$320		Add to Cart
BHE12115439	E2454Ra	96T	$458		Add to Cart

Rat Cystatin-S - CST4 ELISA Kit

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