Rat Cystatin C,Cys-C ELISA Kit

Rat Cystatin C,Cys-C ELISA Kit

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Mfr.No.

CSB-E08385r

Alternative Names

cystatin C, ARMD11, MGC117328, OTTHUMP00000164181, OTTHUMP00000164182, bA218C14.4 (cystatin C), cystatin 3, gamma-trace, neuroendocrine basic polypeptide, post-gamma-globulin

Reactivity

Rat

Detection Range

0.312 ng/ml-20 ng/ml

Sample Type

Serum, Plasma, Homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human ProGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	97	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ProGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	87
	Range %	80-90
1:10	Average %	97
	Range %	92-101
1:20	Average %	98
	Range %	93-102
1:40	Average %	93
	Range %	86-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.432	2.327	2.380	2.253
600	1.894	1.886	1.890	1.763
300	1.271	1.237	1.254	1.127
150	0.721	0.692	0.707	0.580
75	0.467	0.452	0.460	0.333
37.5	0.338	0.345	0.342	0.215
18.75	0.238	0.225	0.232	0.105
0	0.129	0.125	0.127	?

Species

Rattus norvegicus

Sensitivity

0.078 ng/ml

Limit Of Detection

450 nm

UniProt ID	P14841
Antigen	Cystatin C

Applications

ELISA

Research Area

CST3

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507860	CSB-E08385r	96T	$695	Add to Cart
BHE10507860	CSB-E08385r	5x96T	$3336	Add to Cart
BHE10507860	CSB-E08385r	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.