Matrices listed below were spiked with certain level of Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-95
92
EDTA plasma(n=5)
85-100
91
heparin plasma(n=5)
87-100
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Cys-C ELISA Kit allows for the in vitro quantitative determination of Cys-C concentrations in serum, plasma, tissue homogenates and other biological fluids.