Matrices listed below were spiked with certain level of Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-101
95
EDTA plasma(n=5)
87-102
98
heparin plasma(n=5)
89-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-104%
86-104%
93-102%
EDTA plasma(n=5)
82-98%
83-95%
87-99%
heparin plasma(n=5)
83-100%
81-97%
80-94%
Sensitivity
< 1.875ng/ml
Notes
For Research Use Only
UniProt ID
P14841
Applications
ELISA
Applications Notes
Cys-CELISA Kit allows for the in vitro quantitative determination of Cys-C concentrations in serum, plasma, tissue homogenates and other biological fluids.