Rat CYP3A5(Cytochrome P450 3A5) ELISA Kit

Mfr.No.

ELK7419

Alternative Names

CP35; P450PCN3; PCN3; Cytochrome P450,Subfamily IIIA(Niphedipine Oxidase),Polypeptide 5; Cytochrome P450 HLp2

Reactivity

Rat

Detection Range

1.57-100 ng/mL

Sample Type

Cell lysates, Tissue homogenates, Other biological fluids

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant CYP3A5 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP3A5 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	80-93%	86%
Heparin plasma(n=5)	94-105%	98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP3A5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	86-95%	93-103%	87-98%	85-96%
EDTA plasma(n=5)	88-97%	86-102%	91-103%	89-97%
Heparin plasma(n=5)	85-102%	87-96%	82-90%	92-101%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
100.00	2.038	1.952
50.00	1.612	1.526
25.00	1.224	1.138
12.50	0.825	0.739
6.25	0.576	0.490
3.13	0.354	0.268
1.57	0.232	0.146
0.00	0.086	0.000

Sensitivity

0.59 ng/mL

Limit Of Detection

100 ng/mL

Antigen	CYP3A5
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CYP3A5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CYP3A5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CYP3A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CYP3A5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Tumor immunity;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15204622	ELK7419	48T	$320		Add to Cart
BHE15204622	ELK7419	96T	$458		Add to Cart

Rat CYP3A5(Cytochrome P450 3A5) ELISA Kit

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