Rat Cyp27b1(25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) ELISA Kit

Mfr.No.

ER0420

Alternative Names

Cyp27b1 ELISA Kit, Cytochrome P450VD1-alpha ELISA Kit, Cytochrome P450C1 alpha ELISA Kit, Cytochrome P450 subfamily XXVIIB polypeptide 1 ELISA Kit, Calcidiol 1-monooxygenase ELISA Kit, Cytochrome p450 27B1 ELISA Kit, 25-OHD-1 alpha-hydroxylase ELISA Kit, 25-hydroxyvitamin D ELISA Kit, 3 1-alpha-hydroxylase ELISA Kit, VD3 1A hydroxylase ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

0.625-40ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Cyp27b1 and the recovery rates were calculated by comparing the measured value to the expected amount of Cyp27b1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-104	98
EDTA plasma(n=5)	85-104	95
heparin plasma(n=5)	89-102	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cyp27b1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-94%	87-98%	85-98%
EDTA plasma(n=5)	82-95%	87-101%	82-96%
heparin plasma(n=5)	85-98%	86-99%	81-99%

Sensitivity

< 0.375ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Cyp27b1/Cytochrome P450VD1-alpha/Cytochrome P450C1 alpha/Cytochrome P450 subfamily XXVIIB polypeptide 1/Calcidiol 1-monooxygenase/Cytochrome p450 27B1/25-OHD-1 alpha-hydroxylase/25-hydroxyvitamin D(3) 1-alpha-hydroxylase(VD3 1A hydroxylase

Notes

For Research Use Only

UniProt ID	O35132
Antigen	Cyp27b1

Applications	ELISA
Applications Notes	Cyp27b1 ELISA Kit allows for the in vitro quantitative determination of Cyp27b1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10806875	ER0420	96T	$400		Add to Cart

Rat Cyp27b1(25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) ELISA Kit

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