Rat cyclophilin A,CyPA ELISA Kit

Rat cyclophilin A,CyPA ELISA Kit

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Mfr.No.

CSB-E14928r

Sensitivity

31.25 pg/mL

Detection Range

125 pg/mL-8000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

peptidylprolyl isomerase A (cyclophilin A), CYPA, CYPH, MGC117158, MGC12404, MGC23397, PPIase A, T cell cyclophilin, cyclosporin A-binding protein, peptidyl-prolyl cis-trans isomerase A, peptidylprolyl isomerase A, rotamase A

Recovery

The recovery of human SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	97	91-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	85-93
1:2	Average %	91
	Range %	87-98
1:4	Average %	94
	Range %	89-99
1:8	Average %	94
	Range %	82-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
0	2.053	2.137	2.095
12	1.498	1.488	1.493
48	1.086	1.052	1.069
192	0.591	0.620	0.606
600	0.261	0.276	0.269
2400	0.114	0.115	0.115

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P10111

Applications	ELISA
Research Area	PPIA

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.