Rat cyclophilin A,CyPA ELISA Kit

Rat cyclophilin A,CyPA ELISA Kit

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Mfr.No.

CSB-E14928r

Alternative Names

peptidylprolyl isomerase A (cyclophilin A), CYPA, CYPH, MGC117158, MGC12404, MGC23397, PPIase A, T cell cyclophilin, cyclosporin A-binding protein, peptidyl-prolyl cis-trans isomerase A, peptidylprolyl isomerase A, rotamase A

Reactivity

Rat

Detection Range

125 pg/mL-8000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human SRD5A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	97	91-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SRD5A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	85-93
1:2	Average %	91
	Range %	87-98
1:4	Average %	94
	Range %	89-99
1:8	Average %	94
	Range %	82-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
0	2.053	2.137	2.095
12	1.498	1.488	1.493
48	1.086	1.052	1.069
192	0.591	0.620	0.606
600	0.261	0.276	0.269
2400	0.114	0.115	0.115

Species

Rattus norvegicus

Sensitivity

31.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	P10111
Antigen	peptidylprolyl isomerase A (cyclophilin A)

Applications

ELISA

Research Area

PPIA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507857	CSB-E14928r	96T	$595	Add to Cart
BHE10507857	CSB-E14928r	5x96T	$2856	Add to Cart
BHE10507857	CSB-E14928r	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.