Rat cyclooxygenase-2,COX-2 ELISA Kit

Rat cyclooxygenase-2,COX-2 ELISA Kit

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Mfr.No.

CSB-E13399r

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2, cyclooxygenase 2b, prostaglandin G/H synthase and cyclooxygenase, prostaglandin-endoperoxide synthase 2

Recovery

The recovery of bovine IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	89	83-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	92
	Range %	87-97
1:4	Average %	84
	Range %	80-88
1:8	Average %	98
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
4000	0.147	0.141	0.144
2000	0.296	0.279	0.288
1000	0.431	0.467	0.449
500	0.782	0.823	0.803
250	1.862	1.826	1.844

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P35355

Applications	ELISA
Research Area	PTGS2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507856	CSB-E13399r	96T	$695	Add to Cart
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BHE10507856	CSB-E13399r	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.