Rat CXCL1/Gro Alpha ELISA Kit PicoKine®

Mfr.No.

EK0724

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.065	0.098	0.187	0.267	0.428	0.768	1.298	2.038

Description

Rat CXCL1/Gro Alpha ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cxcl1 in cell culture supeatants, serum and plasma (EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth-regulated alpha protein;C-X-C motif chemokine 1;Cytokine-induced neutrophil chemoattractant 1;CINC-1;Platelet-derived growth factor-inducible protein KC;Cxcl1;Cinc1, Gro, Scyb1;

Molecular Weight

10249 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A25-K96

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat CXCL1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	36	226	428	36	217	466
Standard deviation	1.58	9.26	26.1	1.58	9.33	33.55
CV (%)	4.4%	4.1%	6.1%	4.4%	4.3%	7.2%

Protein Function

Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.

Background

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	36	37	34	32	34	1.92	5.6%
Sample 2	226	219	236	214	223	8.25	3.6%
Sample 3	428	378	438	405	412	23.11	5.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	81503
Gene Name	CXCL1
UniProt ID	P14095
Protein Name	Growth-regulated alpha protein
Tissue Specificity	At least expressed in the lung and trachea.

Applications	ELISA
Research Area	Cytokines,G Protein Signaling,Immunology,Innate Immunity,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000588	EK0724	96 wells/kit	$499		Add to Cart

Rat CXCL1/Gro Alpha ELISA Kit PicoKine®

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