Rat CPE/Carboxypeptidase E ELISA Kit PicoKine®

Mfr.No.

EK1889

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.013	0.068	0.119	0.202	0.409	0.787	1.459	2.189

Description

Rat CPE ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cpe in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase

Immunogen

Expression system for standard: NS0; Immunogen sequence: L43-F476

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat CPE

Subcellular Localization

Secreted, secretory vesicle membrane, Peripheral membrane protein

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	72	271	858	81	282	926
Standard deviation	3.96	20.87	39.47	4.70	17.48	63.89
CV (%)	5.5%	7.7%	4.6%	5.8%	6.2%	6.9%

Protein Function

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing.

Background

CPE may play a role in post-translational processing of peptide hormones including preproopiomelanocortin. It is mapped to 16p13.This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	72	65	65	65	66	3.03	4.5%
Sample 2	271	294	289	283	284	8.58	3.0%
Sample 3	858	870	828	820	844	20.63	2.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	25669
Gene Name	Cpe
UniProt ID	P15087
Tissue Specificity	Expressed in brain, heart and anterior pituitary gland. Also expressed in pancreatic islets and adrenal gland.

Applications	ELISA
Research Area	Cell Type Marker,Neuron Marker,Neuroscience,Synapse Marker

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001434	EK1889	96 wells/kit	$750		Add to Cart

Rat CPE/Carboxypeptidase E ELISA Kit PicoKine®

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