Rat Corticosteroid 11-beta-dehydrogenase Isozyme 1

Mfr.No.

E1928Ra

Sensitivity

3.29ng/L

Detection Range

7-1500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

11-beta-HSD1;11-beta-hydroxysteroid dehydrogenase 1;11-DH;Corticosteroid 11-beta-dehydrogenase isozyme 1;HSD11B1

Molecular Weight

31883

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1928Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	103.7	6.21	6
Intra-Assay	2	18	299.5	14.43	4.8
Intra-Assay	3	18	155.3	8.26	5.3

Background

Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone (By similarity). Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol (By similarity).

Protocol

These standard curves of E1928Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.275	2.184	2.142
	2.094
400ng/L	1.928	1.927	1.885
	1.927
200ng/L	1.179	1.16	1.118
	1.141
100ng/L	0.741	0.694	0.652
	0.647
50ng/L	0.402	0.412	0.369
	0.421
0ng/L	0.046	0.042	0
	0.039

Gene ID	25116
UniProt ID	P16232

Research Area	Signal Transduction; Cancer; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113127	E1928Ra	48T	$320		Add to Cart
BHE12113127	E1928Ra	96T	$458		Add to Cart

Rat Corticosteroid 11-beta-dehydrogenase Isozyme 1 - HSD11B1 ELISA Kit

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