Matrices listed below were spiked with certain level of Coq10a and the recovery rates were calculated by comparing the measured value to the expected amount of Coq10a in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-99
94
EDTA plasma(n=5)
90-103
98
heparin plasma(n=5)
85-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Coq10a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-98%
97-104%
89-104%
EDTA plasma(n=5)
84-101%
83-101%
82-95%
heparin plasma(n=5)
85-99%
82-98%
83-97%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
D3ZX74
Applications
ELISA
Applications Notes
Coq10a ELISA Kit allows for the in vitro quantitative determination of Coq10a concentrations in serum, plasma, tissue homogenates and other biological fluids.