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Home / ELISA Kit

Rat Copeptin ELISA kit

SKU : BHE10507829

This Human DES ELISA Kit was designed for the quantitative measurement of Human DES protein in serum, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E16335r

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Copeptin

Recovery

The recovery of human DES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		92		85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		93
		Range %		87-99
1:2		Average %		92
		Range %		87-98
1:4		Average %		88
		Range %		82-92
1:8		Average %		97
		Range %		94-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.932	2.902	2.917	2.827
5	2.173	2.098	2.136	2.046
2.5	1.466	1.421	1.444	1.354
1.25	0.788	0.773	0.781	0.691
0.625	0.462	0.445	0.454	0.364
0.312	0.298	0.279	0.289	0.199
0.156	0.206	0.191	0.199	0.109
0	0.093	0.087	0.090	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

Applications	ELISA
Research Area	copeptin

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Tumour necrosis factor and interleukin 10 in blood pressure regulation in spontaneously hypertensive and normotensive rats AgnieszkaSegiet.et al,Cytokine,July 6

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SKU	Supplier No.	Size	Your price	Quantity
BHE10507829	CSB-E16335r	96T	$695		Add to Cart
BHE10507829	CSB-E16335r	5x96T	$3336		Add to Cart
BHE10507829	CSB-E16335r	10x96T	$6602.50		Add to Cart

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