Rat Connexin 40 - CX40 ELISA Kit

Mfr.No.

E1268Ra

Sensitivity

0.043ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Connexin 40 (also known as CX40) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Connexin-40;Cx40;Gap junction alpha-5 protein;GJA 5;GJA5

Molecular Weight

40233

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1268Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.4	0.53	4.2
Intra-Assay	2	18	2.7	0.22	7.9
Intra-Assay	3	18	7.1	0.48	6.7

Background

One gap junction consists of a cluster of closely packed pairs of transmembrane Channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Protocol

These standard curves of E1268Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.311	2.319	2.273
	2.327
12ng/ml	1.662	1.647	1.6
	1.632
6ng/ml	0.977	1.071	1.025
	1.165
3ng/ml	0.667	0.663	0.616
	0.658
1.5ng/ml	0.432	0.464	0.417
	0.495
0ng/ml	0.032	0.046	0
	0.06

Gene ID	50563
UniProt ID	P28234

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112511	E1268Ra	48T	$320		Add to Cart
BHE12112511	E1268Ra	96T	$458		Add to Cart

Rat Connexin 40 - CX40 ELISA Kit

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