Rat connective tissue growth factor,CTGF ELISA Kit

Rat connective tissue growth factor,CTGF ELISA Kit

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Mfr.No.

CSB-E07876r

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

connective tissue growth factor, CCN2, HCS24, IGFBP8, MGC102839, NOV2, hypertrophic chondrocyte-specific protein 24, insulin-like growth factor-binding protein 8

Recovery

The recovery of mouse AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-93
EDTA plasma (n=4)	98	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-92
1:2	Average %	102
	Range %	95-106
1:4	Average %	93
	Range %	85-96
1:8	Average %	97
	Range %	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.257	2.166	2.212	2.038
10	1.361	1.378	1.370	1.196
5	0.858	0.884	0.871	0.697
2.5	0.572	0.578	0.575	0.401
1.25	0.447	0.433	0.440	0.266
0.625	0.339	0.354	0.347	0.173
0.312	0.248	0.232	0.240	0.066
0	0.176	0.172	0.174

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9R1E9

Applications	ELISA
Research Area	CTGF

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.