Rat Complement Factor D - Adipsin

Mfr.No.

E1114Ra

Sensitivity

0.142ng/ml

Detection Range

0.3-90ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Adipsin (also known as ADIPSIN) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Adipsin;C3 convertase activator;CFD;Complement factor D;Endogenous vascular elastase;Properdin factor D

Molecular Weight

28442

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1114Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.3	0.53	5.8
Intra-Assay	2	18	23.7	1.35	5.7
Intra-Assay	3	18	6.1	0.38	6.2

Background

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alteate pathway. Its function is homologous to that of C1s in the classical pathway.

Protocol

These standard curves of E1114Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.386	2.25	2.209
	2.114
24ng/ml	1.884	1.824	1.783
	1.764
12ng/ml	1.181	1.139	1.098
	1.097
6ng/ml	0.66	0.647	0.606
	0.634
3ng/ml	0.432	0.412	0.371
	0.391
0ng/ml	0.029	0.041	0
	0.053

Gene ID	54249
UniProt ID	P32038

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112362	E1114Ra	48T	$320		Add to Cart
BHE12112362	E1114Ra	96T	$458		Add to Cart

Rat Complement Factor D - Adipsin - CFD ELISA Kit

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