Rat Complement 4,C4 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08706r

Sensitivity

3.12 ng/mL

Detection Range

12.5 ng/mL-800 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Complement 4,C4

Recovery

The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-94
EDTA plasma (n=4)	103	95-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	91-106
1:2	Average %	90
	Range %	85-96
1:4	Average %	93
	Range %	89-100
1:8	Average %	99
	Range %	93-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P08649

Applications	ELISA
Research Area	C4

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity L.V. Fedulova.et al,Journal of Pharmacy and Nutrition Sciences,Apr 25 iTRAQ-based proteomic analysis to identify the molecular mechanism of Zhibai Dihuang Granule in the Yin-deficiency-heat syndrome rats Liu CM.et al,Chin Med,Jan 8

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