Rat Complement 3,C3 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08666r

Sensitivity

0.156 ug/mL

Detection Range

0.625 ug/mL-40 ug/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Complement 3,C3

Recovery

The recovery of mouse TYR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-99
EDTA plasma (n=4)	98	95-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TYR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-99
1:2	Average %	88
	Range %	84-92
1:4	Average %	101
	Range %	98-104
1:8	Average %	86
	Range %	83-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P01026

Applications	ELISA
Research Area	C3

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity L.V. Fedulova.et al,Journal of Pharmacy and Nutrition Sciences,Apr 25

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