Rat Collagen Type III,Col III ELISA KIT

Rat Collagen Type III,Col III ELISA KIT

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Mfr.No.

CSB-E07924r

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Collagen Type III,Col III, Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III, Collagen Type III,Col III, Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III

Recovery

The recovery of human APLNR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	94-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human APLNR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	97
	Range %	94-103
1:8	Average %	101
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.312	2.189	2.251	2.081
750	1.494	1.455	1.475	1.305
375	1.026	0.994	1.010	0.840
187.5	0.655	0.688	0.672	0.502
94	0.448	0.442	0.445	0.275
47	0.303	0.292	0.298	0.128
23.5	0.241	0.232	0.237	0.067
0	0.163	0.177	0.170

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Col III

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Modified XiaochaihuDecoction (加味小柴胡汤) promotes collagen degradation and inhibits pancreatic fibrosis in chronic pancreatitis rats Shu-kun Zhang.et al,Chinese Journal of Integrative Medicine,Nov 28

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.