Rat Collagen Type II,Col II ELISA Kit

Rat Collagen Type II,Col II ELISA Kit

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Mfr.No.

CSB-E08092r

Alternative Names

Collagen Type II,Col II, Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II, Collagen Type II,Col II, Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II

Reactivity

Rat

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human apelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	99	94-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human apelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-104
1:2	Average %	90
	Range %	84-96
1:4	Average %	86
	Range %	82-92
1:8	Average %	101
	Range %	98-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.532	2.432	2.482	2.354
4000	1.904	1.804	1.854	1.726
2000	1.287	1.187	1.237	1.109
1000	0.726	0.716	0.721	0.593
500	0.457	0.447	0.452	0.324
250	0.355	0.345	0.350	0.222
125	0.237	0.227	0.232	0.104
0	0.129	0.127	0.128	?

Species

Rattus norvegicus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

Antigen	Collagen Type Ⅱ,Col Ⅱ

Applications

ELISA

Research Area

Col II

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Mechanical stretch promotes matrix metalloproteinase-2 and prolyl-4-hydroxylase a1 production in human aortic smooth muscle cells via Akt-p38 MAPK-JNK signaling. Liu X et al,Int J Biochem Cell Biol,2015 Feb 21.pii: S1357-2725(15)00052-7. Jatrorrhizine Hydrochloride Suppresses Proliferation, Migration, and Secretion of Synoviocytes In Vitro and Ameliorates Rat Models of Rheumatoid Arthritis In Vivo Haiwen Qiu.et al,Int. J. Mol. Sci.,May 18

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BHE10507799	CSB-E08092r	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.