Rat collagen type II(Col II) antibody ELISA kit

Rat collagen type II(Col II) antibody ELISA kit

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Mfr.No.

CSB-EQ027975RA

Sensitivity

0.078 U/mL

Detection Range

0.312 U/mL-20 U/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

collagen type II(Col II) antibody, collagen type 2(Col 2) antibody,_x000D_ collagen type II(Col II) antibody, collagen type II(Col II) antibody, collagen type 2(Col 2) antibody,_x000D_ collagen type II(Col II) antibody

Recovery

The recovery of human GBP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	98	91-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GBP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	98
	Range %	94-105
1:4	Average %	97
	Range %	93-110
1:8	Average %	94
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	1.794	1.952	1.873	1.778
800	1.175	1.101	1.138	1.043
400	0.634	0.604	0.619	0.524
200	0.351	0.339	0.345	0.250
100	0.275	0.270	0.273	0.178
50	0.195	0.192	0.194	0.099
25	0.142	0.140	0.141	0.046
0	0.095	0.094	0.095	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Col II Ab

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507798	CSB-EQ027975RA	96T	$695	Add to Cart
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BHE10507798	CSB-EQ027975RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.