Rat Cold-inducible RNA-binding Protein

Mfr.No.

E1332Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Cold-inducible RNA-binding Protein (also known as CIRBP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

A18 hnRNP;CIRBP;Cold-inducible RNA-binding protein;Glycine-rich RNA-binding protein CIRP

Reactivity

Rat

Detection Range

5-1000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

18607

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.47ng/L

Background

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor. Promotes assembly of stress granules (SGs), when overexpressed (By similarity).

Gene ID	81825
UniProt ID	P60825
Antigen	CIRBP

Research Area

Epigenetics and Nuclear Signaling; Cell Biology

Protocol

These standard curves of E1332Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.236	2.168	2.111
	2.1
320ng/L	1.543	1.643	1.586
	1.743
160ng/L	1.149	1.102	1.044
	1.055
80ng/L	0.657	0.665	0.607
	0.673
40ng/L	0.472	0.478	0.421
	0.485
0ng/L	0.063	0.057	0
	0.052

Precision

We measured random samples of E1332Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	414.37	16.05	3.87
Intra-Assay	2	18	30.23	2.03	6.71
Intra-Assay	3	18	151.99	11.41	7.51

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112571	E1332Ra	48T	$320		Add to Cart
BHE12112571	E1332Ra	96T	$458		Add to Cart

Rat Cold-inducible RNA-binding Protein - CIRBP ELISA Kit

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