Rat COL4 (Collagen Type IV) ELISA Kit

Mfr.No.

ER0854

Sensitivity

< 0.281ng/ml

Detection Range

0.469-30ng/ml

Reactivity

Rat

Assay Type

Sandwich

Detection Method

Double Antibody

Recovery

Matrices listed below were spiked with certain level of COL4 and the recovery rates were calculated by comparing the measured value to the expected amount of COL4 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	89-104	98
EDTA plasma(n=5)	85-98	92
heparin plasma(n=5)	89-101	97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-99%	87-104%	89-99%
EDTA plasma(n=5)	83-94%	84-99%	83-99%
heparin plasma(n=5)	84-99%	81-98%	82-100%

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Precision

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Background

COL4/Collagen Type Ⅳ/Type-IV Collagen

Notes

For Research Use Only

Applications	ELISA
Applications Notes	COL4 ELISA Kit allows for the in vitro quantitative determination of COL4 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10800229	ER0854	96T	$500		Add to Cart

Rat COL4 (Collagen Type IV) ELISA Kit

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