Matrices listed below were spiked with certain level of Col27a1 and the recovery rates were calculated by comparing the measured value to the expected amount of Col27a1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
96
EDTA plasma(n=5)
87-103
94
heparin plasma(n=5)
89-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Col27a1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Col27a1 chain) ELISA Kit allows for the in vitro quantitative determination of Col27a1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Col27a1(Collagen alpha-1(XXVII) chain) ELISA Kit