Rat coagulation factor X,FX ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08441r

Alternative Names

coagulation factor X, RP11-98F14.9, FX, FXA, Stuart-Prower factor, factor Xa, prothrombinase

Reactivity

Rat

Detection Range

1.56 ng/mL-100 ng/mL

Sample Type

Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human AKT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	82-89
EDTA plasma (n=4)	93	90-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AKT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-99
1:2	Average %	87
	Range %	82-90
1:4	Average %	101
	Range %	98-104
1:8	Average %	90
	Range %	87-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.149	2.169	2.159	2.015
10	1.444	1.469	1.457	1.313
5	0.918	0.938	0.928	0.784
2.5	0.577	0.598	0.588	0.444
1.25	0.388	0.399	0.394	0.250
0.625	0.301	0.321	0.311	0.167
0.312	0.253	0.268	0.261	0.117
0	0.143	0.145	0.144

Species

Rattus norvegicus

Sensitivity

0.39 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q63207
Antigen	Coagulation Factor X

Applications

ELISA

Research Area

F10

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10507783	CSB-E08441r	96T	$856	Add to Cart
BHE10507783	CSB-E08441r	5x96T	$4108.80	Add to Cart
BHE10507783	CSB-E08441r	10x96T	$8132	Add to Cart