Rat Coagulation Factor 10

Mfr.No.

E0490Ra

Sensitivity

0.27ng/mL

Detection Range

0.5-200ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Coagulation Factor Ⅹ (also known as FX) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Activated factor Xa heavy chain;Coagulation factor X;F1;F10;Factor X heavy chain;Factor X light chain;Stuart factor

Molecular Weight

54265

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0490Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	52	1.5	2.9
Intra-Assay	2	18	23.4	1.07	4.6
Intra-Assay	3	18	32.1	1.29	4

Background

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

Protocol

These standard curves of E0490Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/mL	2.039	2.057	2.008
	2.075
60ng/mL	1.437	1.5	1.451
	1.563
30ng/mL	0.77	0.717	0.668
	0.664
15ng/mL	0.462	0.393	0.344
	0.324
7.5ng/mL	0.205	0.204	0.154
	0.202
0ng/mL	0.051	0.049	0
	0.047

Gene ID	29243
UniProt ID	Q63207

Research Area	Blood Coagulation

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111777	E0490Ra	48T	$320		Add to Cart
BHE12111777	E0490Ra	96T	$458		Add to Cart

Rat Coagulation Factor 10 - F10 ELISA Kit

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