Rat Cluster of Differentiation 68, CD68 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13297r

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Cluster of Differentiation 68, CD68

Recovery

The recovery of mouse TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	88	83-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	87
	Range %	82-91
1:10	Average %	104
	Range %	100-108
1:20	Average %	99
	Range %	96-105
1:40	Average %	91
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q4FZY1

Applications	ELISA
Research Area	CD68

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Hypothermic oxygenated perfusion (HOPE) protects from biliary injury in a rodent model of DCD liver transplantation Andrea Schlegel et al,Journal of Hepatology,2013

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