Rat Cluster of Differentiation 63(CD63) ELISA Kit

Rat Cluster of Differentiation 63(CD63) ELISA Kit

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Mfr.No.

CSB-E14117r

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

CD63 molecule, LAMP-3, ME491, MLA1, OMA81H, TSPAN30, CD63 antigen, CD63 antigen (melanoma 1 antigen), granulophysin, lysosome-associated membrane glycoprotein 3, melanoma 1 antigen, melanoma-associated antigen ME491, me

Recovery

The recovery of rat PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-105
1:2	Average %	94
	Range %	91-105
1:4	Average %	95
	Range %	90-101
1:8	Average %	97
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
50	0.147	0.146	0.147
9.37	0.305	0.314	0.310
1.56	0.512	0.523	0.518
0.44	0.865	0.887	0.876
0.125	1.411	1.424	1.418
0	2.118	2.122	2.120

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P28648

Applications	ELISA
Research Area	CD63

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507778	CSB-E14117r	96T	$685	Add to Cart
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BHE10507778	CSB-E14117r	10x96T	$6507.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.