Rat cluster of differentiation 4,CD4 ELISA Kit

Mfr.No.

CSB-E12928r

Sensitivity

0.195 U/mL

Detection Range

0.78 U/mL-50 U/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

CD4 molecule, CD4mut, CD4 antigen, CD4 antigen (p55), CD4 receptor

Recovery

The recovery of rat TPH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	97	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TPH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-102
1:2	Average %	94
	Range %	87-98
1:4	Average %	102
	Range %	96-105
1:8	Average %	91
	Range %	87-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P05540

Applications	ELISA
Research Area	CD4

Storage	Store at 2-8°C. Please refer to protocol.

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