Rat circulating immune complex(CIC)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E17938r

Alternative Names

circulating immune complex(CIC)

Reactivity

Rat

Detection Range

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Assay Duration

1-5h

Recovery

The recovery of human FAM20A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-89
EDTA plasma (n=4)	100	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FAM20A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	87
	Range %	82-91
1:4	Average %	99
	Range %	94-107
1:8	Average %	90
	Range %	85-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

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Limit Of Detection

450 nm

Antigen	circulating immune complex(CIC)

Applications

ELISA

Research Area

CIC

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity L.V. Fedulova.et al,Journal of Pharmacy and Nutrition Sciences,Apr 25

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